Fluent® Gx Automation Workstation

Fluent® Gx Automation Workstation

₦4000.00

Tecan Trading AG

In eukaryotes DNA generally is methylated on cytosines in the context of CpG dinucleotides – a modification called 5-methylcytosine (5-mC). More recently, researchers have discovered that cytosines can also be h...

In eukaryotes DNA generally is methylated on cytosines in the context of CpG dinucleotides – a modification called 5-methylcytosine (5-mC). More recently, researchers have discovered that cytosines can also be hydromethylated (5-hmC), formylated (5-fC) and carboxylated (5-caC). 

Researchers can purify methylated DNA using either antibodies or recombinant methyl-binding proteins (MBDs), methods that sometimes are called methyl-DNA immunoprecipitation (MeDIP) and the Methylated DNA Island Recover Assay (MIRA), respectively. 

To differentiate standard cytosine from its various modified forms, researchers have several options. Some restriction enzymes can distinguish methylated and unmethylated CpGs. Or, they can use sodium bisulfite to convert cytosine (but not 5-mC or 5-hmC) to uracil and sequence the resulting nucleic acids (bisulfite sequencing). More recently, tools have been developed to differentiate 5-mC- and 5-hmC-containing nucleic acids (which bisulfite cannot do), such as the next-gen sequencing methods, TAB-Seq and OxBS-Seq. 

In eukaryotes DNA generally is methylated on cytosines in the context of CpG dinucleotides – a modification called 5-methylcytosine (5-mC). More recently, researchers have discovered that cytosines can also be hydromethylated (5-hmC), formylated (5-fC) and carboxylated (5-caC). 

Researchers can purify methylated DNA using either antibodies or recombinant methyl-binding proteins (MBDs), methods that sometimes are called methyl-DNA immunoprecipitation (MeDIP) and the Methylated DNA Island Recover Assay (MIRA), respectively. 

To differentiate standard cytosine from its various modified forms, researchers have several options. Some restriction enzymes can distinguish methylated and unmethylated CpGs. Or, they can use sodium bisulfite to convert cytosine (but not 5-mC or 5-hmC) to uracil and sequence the resulting nucleic acids (bisulfite sequencing). More recently, tools have been developed to differentiate 5-mC- and 5-hmC-containing nucleic acids (which bisulfite cannot do), such as the next-gen sequencing methods, TAB-Seq and OxBS-Seq. 

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Fluent® Gx Automation Workstation

In eukaryotes DNA generally is methyl...